Discussion:
Calculations for Determining a Titer
The purpose of the experiment that took place was to determine the titer of B-D-galactosidase using the enzyme-linked immunosorbent assay. I used a formula obtained from class mates to find the titer. I want to be sure this is the correct method for determining the titer. I attached my data, including the results from the plate reader, and my graphs of the log of the inverse of the antibody dilution vs. absorbance. The formula I used to find the titer of rows C and D are below. If this is not the correct method for finding the titer, what is, and how is it performed? And what do the results of the titers mean?
Row C
y = -0.033x + 0.1864
A450 max = 0.263
½ max = 0.1315
0.1315 = -0.033x + 0.1864
-0.0549 = -0.033x
1.6636 = x
10^(1.6636) = 46.09
Titer = 46.09
Row D
y = -0.0415x + 0.2306
A450 max = 0.339
½ max = 0.1695
0.1695 = -0.0415x + 0.2306
-0.0611 = -0.0415x
1.4723 = x
10^(1.4723) = 29.67
Titer = 29.67