Assignment:
Acetic acid is a good choice to make buffers that are moderately acidic because it is easy to use, inexpensive, and the ions do not interact strongly with most biomolecules. The pKa of acetic acid is 4.75. We want to prepare 1.5 L of buffer at pH = 5.25 that is approximately 50 mM in concentration. You have the following solutions available to use (and only these):
0,050 M acetic acid
2.0 M NaOH
2.0 M HCl
What will you mix using the above stock solutions to make your buffer? Before you begin any calculations, explain your strategy and any assumptions you make. After you calculate the required volumes, calculate the actual final pH, volume, and concentration of each species in solution. (Hint: There should be five solutes present at equilibrium).
Provide complete and step by step solution for the question and show calculations and use formulas.