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Based on the equation below which ion plays the role of

Based on the equation below, which ion plays the role of hydrogen-ion donor (acid) and which ion plays the role of hydrogen-ion acceptor (base) in PBS?

H2PO4-(aq) H+(aq) + HPO42-(aq)

The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.027M KCl, pH 7.4. Below is the protocol to make 1 liter of 10x concentrate PBS.

Combine the following:

80g NaCl

2g KCl

14.4g Na2HPO4 (dibasic anhydrous)

2.4g KH2PO4 (monobasic anhydrous)

800mL distilled H2O

Adjust pH to 7.4 with HCl

Add H2O to 1L

Autoclave for 20 minutes on liquid cycle. Store at room temperature.

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