1- The genes for both the alpha-and beta-globin chains of hemoglobin contain introns (i.e., they are split genes). How would this fact affect your plans if you wanted to introduce the gene for alpha-globin into a bacterial plasmid and have the bacteria produce alpha-globin?
2- Bacteria and yeast are known not to have prions. What does this fact have to do with the popularity of expressing mammalian proteins using bacterial vectors?