1) You have 100 ml of a solution of ATP. You take 50 µl, and dilute it to a final volume of 2000 µl. The absorbance of the diluted solution at 259 nm is 0.520.
a) What is the ATP concentration in the original, undiluted solution? (5 points) The molar extinction coefficient for ATP at 259 nm is 15,600 l mol-1cm-1.
b) How many mg of ATP are there in total in the original, undiluted solution? The formula weight of ATP is 551.14 g/mole.