--%>
Assignment Help - homework help

PCR and its working

What is PCR and explain its working?

E

Expert

Verified

PCR, polymerase chain reaction, refers to the method which synthesizes several copies of the specific regions of DNA molecule called as target-regions.

First, DNA to be tested gets heated causing in the double helix to burst and the polynucleotide chains to get exposed. Then the small synthetic sequences of the DNA called as the primers and consisting of nucleotide sequences same as that of the sequences of extremities of the region to be studied are added. The primers that are paired along with the original DNA in extremities of the gene to be amplified. Enzymes termed as polymerases, which catalyze the DNA replication, and the nucleotide supply is added. The primers then are completed and chosen region is replicated. In presence of the more primers and more nucleotides millions of copies of that specific region are produced.

   Related Questions in Biology

  • Q : Managing Gender Diversity -

    There are many ways of managing this. One way is to recognize that there are strengths in both the sexes. Men can team from women's managerial style by using appropriate relationship-building in the workplace, while women can learn from men's style to be less subtle and more assertive and direct,

    		•
  • Q : Line or staff organization Line or

    Line or staff organization The line or staff organization refers to an organization in which two types of authority relationships coexist. They are direct or line au

    		•
  • Q : Types of respiration Explain three

    Explain three types of respiration where the circulatory system transports gases?

    		•
  • Q : Explanation of cellular regeneration

    Give a brief explanation of cellular regeneration. How mitosis is associated to this process?

    		•
  • Q : Goals - Motivation - Organizational

      These are known as the sought after results of motivated behavior. That is, they are the end points of motivation behavior. Goals may be generic or specific. If you are thirsty you may opt for any liquid or you may want a specific brand of drink. Selection of goals<

    		•
  • Q : What is melanocytes What is meant by

    What is meant by the melanocytes?

    		•
  • Q : Perception and Communication One of my

    One of my friend has a problem with perception and communication in Interpersonal communication. Describe the three guidelines for enhancing perception and communication.

    		•
  • Q : Define Systems Security Systems

    Systems Security: It is the control and access of computer system's resources, the information and operating system files. This can be managed by security software installed in the computer. The other meaning might refer to any type of system which se

    		•
  • Q : Antigen-presenting cells of immune

    Specify some of the antigen-presenting cells of immune system?

    		•
  • Q : Define null cells Define null cells.

    Define null cells. Answer: The lymphocytes which are devoid of markers for T and B-cells are termed as null cells.   

    		•