Merits of indirect immuno fluorescence
What are the merits of indirect immuno fluorescence?
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The primary does not require to be conjugated with label.
It raises the sensitivity of staining since multiple fluoro-chrome reagents will join to each antibody molecule. This technique consists of great flexibility.
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What are the demerits of direct immuno fluorescence? Answer: The individual fluorescent conjugate has to be made against each and every antigen to be tested.
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