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Dosage-route of administration making substance antigenic

Briefly explain regarding dosage and route of administration that make a substance antigenic.

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Grouping of optical dosage and routes of administration will persuade a peak immune response in a given animal. The insufficient dose will not stimulate an immune response. The extreme dose does not provide a peak immune response since it causes a state of immunological unresponsiveness or non-response termed as immunological tolerance.   

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