What would be an alternative explanation, Chemistry

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What would be an alternative explanation

You have a 25kD histidine tagged bacterial protein (100% pure).You apply this pure protein to a Ni2+-agarose column. Then you apply crude mouse cell extract to the column. After extensivewashing, you apply an imidizole buffer. Analysis of the resultantelution on an SDS-PAGE gel indicates two proteins (25kD and 74kD)of equalcoomassie blue staining.

1) What is the simplest explanation for why the 25kD and the75kDpurified off the column?

2) What would be an alternative explanation?

3) What control experiment could you perform to distinguish between the two possible conclusions you stated above?

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Chemistry: What would be an alternative explanation

Reference No:- TGS0749405

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Recent Questions Asked Chemistry

Q : Calculate the heat of combustion of one gram

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Request for Solution File

Ask an Expert for Answer!!

Chemistry: What would be an alternative explanation

Reference No:- TGS0749405

Recent Questions Asked Chemistry

Q : Calculate the heat of combustion of one gram

Q : Point estimate for the population standard deviation

Q : Determine the final voltage on capacitor

Q : Find rate of heat transfer and heat transfer surface area

Q : What would be an alternative explanation

Q : Determine cold water and hot water outlet temperatures

Q : Finding the total displacement of the boat

Q : Estimate minimum collector area required for shaft power

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