Problem:
You prepare bacterial cell extracts by lysing the cells and removing insoluble debris via centrifugation. These extracts provide all the protiens required for DNA repication. The DNA template is a small, double-stranded circular piece of DNA (a plasmid) with a single origin of replication and a single replication termination site. The termination site is on the opposite side of the plasmid from the origin. Replication proceeds in both directions from the origin.
In addition to the extracts and the and the plasmid DNA,
Required:
Question: Are there any additional materials you should add to this in vitro replication system? Explain.
Does anyone know how to go about this search to explain it.