Explain studying and predicting the results of an experiment conducted with a mutant mouse.
A researcher newly discovered a mutant mouse with an interesting T cell phenotype. He named this new mutation: HD," bred the mouse as well as then used these founders to generate a strain homozygous for HD. Several of his finding are summarized in the table below:
|
|
Thymocytes
|
Lymph node t cells
|
|
|
CD4+8+
|
CD4+
|
CD8+
|
CD4+
|
CD8+
|
|
Wild typ
|
100
|
40
|
20
|
20
|
12
|
|
HD mutant
|
100
|
1
|
20
|
0.5
|
14
|
1. Explain what he found.
2. He next made bone core chimeras, injecting bone marrow from HD mice into lethally irradiated wild type mice. They originate that thymocytes in these chimeras exhibited a staining pattern identical to that of HD mice. In contrast when they did the inverse, they originate that the thymocytes exhibited a normalCD4/CD8 phenotype. What do these outcomes tell you?
3. Founded on these results advance a hypothesis about the cause of the phenotype.
4. Founded on your hypothesis what do you predict would happen to developing T cell in an HD mouse that has been made transgenic for a TCR restricted to I- Ab. Describe your reasoning.