Q1. Explain the patterns of DNA methylation all through the genome.
Q2. Name the inhibitors of DNA methylation.
Q3. Describe the impact of DNA methylation on gene transcription.
Q4. Explain the methods used to study the DNA methylation.
Journal Article:
M. Esteller, "Cancer Epigenomics: DNA Methylomes and Histone-Modification Maps," Nature Review Genetics, 8 (4, April 2007): 286-98.
Discussion Assignment:
Choose one of the methods explained by Dr. Esteller in the assigned journal article. First, give a short explanation of the method in your own words and then answer the given questions.
A) When or why would you use this method over the other methods? Ask yourself the given questions:
- What are you looking for?
- What are your starting materials?
- Are there time, labor and other cost considerations?
- What do you expect to find?
B) Mention the strengths and drawbacks of this process?