1) Why is the antibiotic ampicillin important for plasmid transformation? ?What's the antibiotic doing, and what if it isn't there following transformation?
2) Which phenotype(s) of E.coli does pUC19 affect? Which phenotype(s) does pGFP affect? Which controls in your experiment allow you to be certain of these claims?
3) What was your hypothesis regarding the effect of DNA form on transformation? Is the form of the plasmid
4) What was your hypothesis regarding arabinose? What effect does arabinose have on cells that contain pUC19? What effect does it have on cells that contain pGFP? Does the concentration of arabinose have any effect on these phenotypes?
5) Many transgenic organisms have been created that contain the GFP gene. Based on your observations here, is expression of the green fluorescent protein harmful to cells? Which observations led you to your answer?