Problem:
Question 1: What role did Restriction Enzymes and DNA ligase have in making the PGLO plasmid. What is meant by expression vectors?
Question 2: What information needs to be in a successful expression vector?
Question 3: What is the role of restriction enzymes in nature?
Question 4: Nucleic acids can be separated by gel electrophoresis. You should understand the steps involved in this process (i.e., "cut and run") as well as be able to interpret results if looking at a gel.
Question 5: What is the difference between DNA and cDNA? Why make cDNA? What are DNA and cDNA libraries?
Please provide description of your answers.