Problem:
I was a fool and dissolved my antibiotic (Kanamycin) into Tris Buffer rather than H?O. The Kanamycin still seems to be active but a fellow labmate mentioned that Tris messes around with the membrane permeability of cells. I was curious to what extent this actually influences experiments.
Tris(hydroxymethyl)aminomethane buffer modification of Escherichia coli outer membrane permeability. Please explain the experiment.