Enzyme Kinetics and the Michaelis-Menten Equation
1. A new enzyme you are studying has a Km of 7.0mM and a kcat of 126 per second. What will be the initial rate of reaction in units mM/min when 9.3 nanometer enzyme is reacted with 2.7 mM substrate. Report your answer in units if mM/min to nearest 1mM/min?
2. A new enzyme you are studying has a Km of 12.6 mM and a kcat of 122 per second. 14.3 nanomolar enzyme is reacted with 40.7 mM substrate. A competitive inhibitor is included on the reaction. The inhibitor is present at 9.1mM and has a Ki of 9.5 mM. What will be the initial rate of reaction in units mM /min to the nearest 1 mM/min?