The resolving power of the immuno diffusion was hugely improved by immuno electrophoresis. This engrosses the electrophoretic division of antigen in its component proteins followed through immuno diffusion.

This method is carried out on one percent agarose gel. Antigen mixture is first electrophori zed and alienated based on charge, troughs are then cut in agarose gel, as well as antiserum is added to troughs.

The agarose gel is then hatched 18 to 24hrs throughout which the antigen and antibody diffuse towards each other. The development of precipitin bands can be observed for individual antigen constituents.